FBI Director Christopher Wray has launched a staunch defence of the agency following criticism by US President Donald Trump.
Mr Wray told a committee of lawmakers that there was "no finer institution".
Mr Trump recently lashed out at the federal law enforcement agency, saying its reputation was "in tatters".
Special Counsel Robert Mueller, a former FBI director, is investigating alleged Russian interference in the US election to help elect Mr Trump.
Mr Trump denies that his team colluded with Moscow.
"There is no finer institution than the FBI and no finer people than the men and women who work there and are its very beating heart," Mr Wray told the House of Representatives judiciary committee.
"The FBI that I see is tens of thousands of brave men and women working as hard as they can to keep people they will never know safe."
He described the bureau's staff as "decent people committed to the highest principles of integrity and professionalism".
Impartiality of investigation under spotlight
Analysis by Anthony Zurcher, BBC News, Washington
FBI Director Christopher Wray was the man testifying before the committee on Thursday morning, but - at least for the Republican congressmen present - it was special counsel Robert Mueller in the spotlight.
In particular, the Republicans honed in on a former member of Mr Mueller's team, Peter Strzok, a top FBI counterintelligence officer removed from the investigation this summer after the agency discovered he had written negative text messages about Donald Trump during the 2016 presidential campaign.
The strategy is clear - to raise doubts about the impartiality of Mr Mueller's investigation. The president's supporters for months have been pointing to perceived Democratic leanings of some of the investigative team, including a handful of donations made to Democratic candidates.
Mr Strzok - who participated in the interview of Trump confidante Michael Flynn as well as the investigation into Hillary Clinton's use of a private email server as secretary of state - has now become the most-cited example of possible bias.
Conservative officeholders and commentators are steadily sharpening their criticisms of Mr Mueller, a former Republican-appointed head of the FBI. As his investigation proceeds, and the list of presidential associates facing criminal charges grows, this partisan pressure is sure to mount.
Republican committee members pressed Mr Wray on alleged bias within the FBI.
He responded by saying that all organisations made mistakes but "independent processes" at the FBI would look into the facts surrounding any mistakes and FBI employees would be held accountable if "appropriate".
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Reports emerged last weekend that Mr Mueller had dismissed an FBI officer from the investigation after he was discovered to have made anti-Trump remarks in text messages.
The president seized on the officer's dismissal, and in a tirade on Twitter he said the FBI's "reputation is in Tatters - worst in history! But fear not, we will bring it back to greatness".
President Trump also issued a fresh denial that he asked former FBI director James Comey to drop an investigation into the conduct of Michael Flynn.
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